Drug Development Cluster

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Circulating Biomarker Core

Mission

To offer circulating biomarker testing services for bench research, clinical trials and clinical grade testing for the University of Wisconsin research community and outside facilities

Services

  • Development and translation of circulating biomarker methods from basic science to clinical research and clinical laboratory diagnostics
  • Custom method development, clinical trial sample processing, CLIA/CAP implementation for circulating biomarker end-points involving circulating tumor cell (CTC) protein or mRNA/DNA evaluation, cell-free DNA, exosomes, etc.
  • Access donated blood from patients with solid tumors of the prostate, lung, bladder, kidney and breast
  • Biospecimen team support for enrolling new patients and coordinating sample acquisition
  • Skilled staff available for all aspects of sample handling: blood draw, processing, and analysis
  • Experienced support for all sample processing aspects of large-scale, multi-site clinical trials
  • Expert understanding of CLIA requirements for clinical laboratory diagnostic preparation
  • Lab space in both WIMR and SLH for convenient consultations and CAP-approved capabilities
  • Free consultation work and options for service structures that operate through fee-for-service or collaborative grant budgets

Equipment

  • Automated sample processing technology (exclusion-based sample preparation (ESP)) demonstrating high levels of precision and accuracy
  • Three total five channel inverted fluorescence microscopes each with automated XYZ stage and perfect focus systems (PFS) from Nikon
  • Quantitative PCR machine (Bio-Rad)

Contact

Drug Development Core

Mission

Our mission is to drive translational drug discovery and development through the discovery, the design, and the synthesis of novel small molecule-based therapeutics.  Additionally, the DDC provides support for bioanalytical assay development, validation and performance in support of clinical and translational research.  The DDC is comprised of a Medicinal Chemistry Center, Small Molecule Screening Center, and a Cancer Pharmacology Lab.

Small Molecule Screening Center (SMSF, Faculty Director: Tim Bugni)

Services

  • HTS SErvices
  • Specialized Libraries
  • Diversity Libraries
  • Assay Ready Plates
  • Mechanisms of Action Assays
  • Seahorse Metabolism Analysis
  • Mycoplasma Detection
  • Modeling & Virtual Screening
  • Preclinical Services

Equipment

  • Formulatrix Tempest, contact free dispenser
  • BlueCat bio, Blue washer, contact free plate washer for 96, 384 and 1536 well plates
  • Benchtop Pipetter 96 and Benchtop Pipetter 96-low volume
  • Biotek Microflo peristaltic reagent dispensers
  • ThermoFisher, CombiDrop
  • BMG Pherastar Multimode plate reader
  • LI-COR Odyssey imaging system (scanner)
  • PerkinElmer Enspire plate reader for AlphaScreen
  • BMG Clariostar plate reader
  • Wallac/PE 1420 VICTOR3 V plate reader
  • Beckman fX Liquid Handling (96 and 384)
  • Labcyte Echo 550

Medicinal Chemistry Center (MCC, Faculty Director: Weiping Tang)

Services:

  • Providing expert opinion to medical researchers seeking medicinal chemistry guidance
  • Synthesis of small molecules that have been identified as bioactive
  • Development of lead and probe molecules for target identification
  • Hit-to-lead optimization to improve drug-like properties
  • Construction of libraries of small molecules, synthetic and naturally occurring, unique to UW-Madison
  • Large-scale preparative synthesis to support animal model studies
  • Chemoinformatic analysis and rational drug design
  • Support for individual and center-based research grants
  • ADME and PK studies through CRO partners

Equipment:

  • Agilent 1290 UHPLC with 6120 single quadrupole MS (multimode ESI and APCI source)
  • Agilent 1200 HPLC (analytical and semi-preparative)
  • Anton Paar Monowave 300 microwave synthesizer
  • Anton Paar Multiwave Pro microwave synthesizer
  • Teledyne Isco CombiFlash Next Gen 300+ with ELSD
  • Teledyne Isco EZ-Prep with PurIon ESI MS and autosampler
  • Waters Autopure prep HPLC system
  • Nanotemper Monolith NT.115 microscale thermophoresis instrument
  • Nicoya Lifesciences OpenSPR instrument
  • Genevac EZ-2 Elite
  • Buchi Rotovapor R-300
  • Chiral analytical and prep columns: RegisPack, RegisCell, Whelk-O1

Cancer Pharmacology Lab (CPL, Faculty Director: Natalie Schmitz)

  • Sample Acquisition Services to support clinical trials
  • Advice on appropriate experimental design
  • Problem definition and statistical methodology
  • Definition of study endpoints
  • Sample size requirements
  • Determination of randomization and stratification
  • Development of systems for quality control, progress reports, and presentation of interim analyses
  • Pharmacokinetic Assays
  • Pharmacodynamic Assays
  • Preclinical Analytic Services

Equipment

  • Agilent 1100 Series LC/MS with diode array detector (DAD)
  • 2 Shimadzu Prominence HPLC systems with fluorescence and UV detectors, one with DAD
  • NanodropND-1000 Spectrophotometer
  • SpectraMax M3 Micro-Plate Reader
  • Cellometer Auto 2000 Cell Viability Counter

Contact

 

Radiopharmaceutical Production Facility

Mission

To develop and manufacture high-quality standard and novel radiopharmaceuticals, in order to support and advance preclinical and clinical translational PET molecular imaging and radiotherapy research and clinical trial services. Our goal is to provide this in an environment emphasizing safety and reliability, insuring a high level of quality in compliance with current federal regulations and guidelines.

Services

  • Radiopharmaceutical Production
  • Radionuclide labeling services

Equipment

  • Gas Chromatograph
  • HPLC
  • Chromatographs
  • endotoxin test, sterility test
  • dose calibrator
  • multi-channel analyzer
  • USP <825> compliant aseptic processing for radiopharmaceutical dispensing

Contact

֍  Utilizes iLab Management System