Cell Biology Cluster

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Flow Cytometry Laboratory ֍

Mission
The mission of the University of Wisconsin Carbone Cancer Center Flow Lab is to maintain a cutting-edge facility that provides researchers the technical and educational support for fluorescence-based single cell analysis and isolation to further the characterization and understanding of cellular function, biomarkers, pathology and treatment in basic, translational, and clinical research projects. The Flow Lab has 2 locations; 7016 WIMR, and 2360 Biotech, as well as a computer lab located in K4/519 of the Clinical Sciences Center.

֍ This core facility utilizes iLab, an online management tool that allows researchers to request services, schedule equipment and manage billing with UW shared resources.

Services

  • Flow Cytometry
  • Image Cytometry
  • Spectral Cytometry
  • Cell Sorting
  • Protocol Development
  • Panel Design
  • Data Analysis
  • Training and Education
  • Assay Services
  • Consultation

Equipment

  • 2 ThermoFisher Attune NxT Flow Cytometer Cytometers (WIMR)
  • 1 BD LSRII Flow Cytometer (WIMR)
  • 1 BD LSR Fortessa Flow Cytometer (WIMR)
  • 1 Cytek Aurora Spectral Cytometer (WIMR)
  • 1 Luminex ImageStreamX MarkII Imaging Cytometer (WIMR)
  • 2 BD FACS Aria Cell Sorters in dedicated Biological Safety Cabinets (WIMR)
  • 1 Nexcelom Cellometer T4 automated cell counter (WIMR)
  • 1 ThermoFisher Attune Nxt V6 Analysis Cytometer (Biotech)
  • 1 BD FACS Aria Cell Sorter in BioBubble (Biotech)
  • 1 PC Data Analysis Computer  equipped with FlowJo, FCS Express, IDEAS, SpectroFlo, MS Office, Adobe Illustrator, Photoshop, MatLab, R, and ModFit  (Biotech)
  • 6 PC computers equipped with FlowJo, FCS Express, IDEAS, SpectroFlo, MS Office, Adobe Illustrator, Photoshop, MatLab, R, and ModFit (CSC)

Not on-line yet, but installed in the SAIRF under Flow Lab management

  • 1 ThermoFisher Attune Nxt Flow Cytometer, reserved for Radiotherapy Research Samples.  Restrictions apply. Please contact the Flow Lab for more information.

Contact

Human Proteomics Program Mass Spectrometry Facility

Mission

The overall mission of the University of Wisconsin-Madison Human Proteomics Program is to improve the health of the citizens of Wisconsin, the nation and the world through a program of research and education dedicated to understanding the basis for human health and disease, identifying potential molecular targets for the treatment of disease, and developing high-throughput assays for early detection of disease and the risk for disease.

Services

Top-down Proteomics

  • Liquid chromatography separation of intact proteins
  • Intact protein accurate mass measurement
  • Identification and quantification of post-translational modification (PTM)
  • Exploration of interdependency between different modification sites
  • Identification of interacting partners
  • Top-down proteomics for precision medicine

Middle-down/Bottom-up Proteomics

  • Partially digestion of proteins using a variety of protease
  • Comprehensive characterization of monoclonal antibody (mAb) and antibody drug conjugate (ADC)
  • Precise determination of protein boundaries for structural biology
  • In-gel or in-solution digestion for protein identification
  • Label-free proteomics for biomarker discovery
  • Discovery of novel modification sites

Metabolomics/Lipidomics

  • Targeted or untargeted metabolomics
  • Label-free metabolomics for biomarker discovery
  • High-resolution MS-based lipidomics
  • Small molecule drug discovery
  • Natural compound fine structure determination

Equipment

  • Bruker SolariX XR FT-ICR Mass Spectrometer
  • Bruker Impact II Q-TOF Mass Spectrometer
  • Bruker maXis II Q-TOF Mass Spectrometer
  • TriVersa NanoMate nanoESI System
  • Waters ACQUITY UPLC M-Class System
  • Waters ACQUITY UPLC H-Class System
  • Waters ACQUITY UPLC Systems with 2D LC Technology

Contact

Skin Disease Research Center

Mission

Establish and maintain a cost-effective one-stop facility as a reliable source of quality-assured cells and cell cultures from normal and pathological human skin specimens and mouse models of skin diseases.

Services

  • Preparation for primary cells and established cell lines for both human and mouse cells
  • iPSC generation
  • 3-D Cultures
  • Lentivirus production and Electroporation
  • Mycoplasma testing with a 24-hour turnaround time
  • Specialty cell culture medium preparation
  • Cell culture training for lentivirus and electroporation productions only

Contact