We are pleased to announce the new SMPH Core Facilities website!
This website is a repository of information describing in detail SMPH Core Facilities and the services available for intramural and extramural use. Each core facility lists specific instrumentation, technologies, consultation services, and other services that scientific and clinical investigators have access to.
Our future goal is for this site to be available to external customers to provide access to our service core infrastructure and to specialized services.
Please click on the core cluster name for more details on the resources and expertise available in each.
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February 2021 - What's New! Lightsheets for Live Cell Imaging: 3i Lattice Lightsheet & Mizar Tilt at the Optical Imaging Core
The 3i Lattice Lightsheet utilizes Bessel beam lattice light sheet illumination. Lightsheet imaging offers fast fluorescence imaging with decreased light dose at the sample plane compared to confocal imaging. Conventional selective plane illumination microscopy (SPIM) methods use a Gaussian beam where light sheet thickness is proportional to light sheet length, leading to a beam that is typically too short or thick for ideal sub-cellular imaging.
The innovative 3i Lattice LightSheet combines the flexibility of a Gaussian light sheet with the thin optical sectioning of a Bessel beam to achieve the best optical sectioning of any light sheet microscope. This enables high spatiotemporal resolution 4D live cell imaging while reducing phototoxicity and photobleaching. The setup is equipped with a Hamamatsu ORCA-Flash4.0 V3 sCMOS high-resolution camera and features a controlled temperature and CO2 incubator for live-cell imaging. This unit is equipped with 488, 560 and 642 excitation lasers and a 25x/1.1NA water immersion objective.
Mizar Imaging’s Tilt is a modular light sheet illumination system enabling high-speed, high-resolution, and 3D light sheet imaging on the Leica DMi8 inverted microscope. It is equipped with 488, 561 and 647 emission lasers, it is ideal for high-resolution imaging while reducing photobleaching and phototoxicity. The setup features a high-resolution Prime 95B camera, and a Leica color camera and the following objective lenses: 5X, 10X, 20x, 40x, 60x, and 100x. This system also includes an environmental chamber for live-cell imaging.
If you are interested in the 3i Lattice Lightsheet or the Mizar Tilt , and have questions or need additional information, please contact the Optical Imaging Core manager, Lance Rodenkirch at firstname.lastname@example.org or learn about other equipment and services provided by this core at https://opticalcore.wisc.edu/microscopes/ .
November 2020 - What's New! MILabs U-SPECT/CTUHR (ultra high-resolution) system at SAIRF
The Small Animal Imaging & Radiotherapy Facility (SAIRF) in conjunction with the generous matching funds from Radiology, Human Oncology, Medical Physics, UWCCC, SMPH, was able to purchase the MILabs U-SPECT/CTUHR (ultra high-resolution) system.
This system will be the only micro-SPECT system in Wisconsin, allows for scanning animals up to the size of a large rat. Single Photon Emission Computed Tomography (SPECT) is a functional imaging technique that detects gamma rays resulting from radioactive decay from an injected radioisotope. A radioisotope can be attached to a specific ligand that can then seek out and bind to a target biological marker. The U-SPECT from MILabs allows imaging of both standard and high-energy theranostic radioisotopes that are commonly used in brain, heart, bone and cancer applications, although many other uses exist as well. Computed Tomography (CT) is traditionally an anatomical imaging technique that uses x-rays to detect density. As such, CT is commonly used in bone applications, but it is also useful for lung and soft tissue applications when a contrast agent is administered. The MILabs CTUHR can achieve a resolution down to 4microns; a major upgrade to our current capabilities which are limited to 50microns.